The 12 PIO MPs voted along party lines.
Voting against deal were Sir Amess MP for Southend West, James Duddridge MP for Rochford and Southend East, Mark Francois MP for Wickford and Rayleigh, Stephen Metcalfe MP for for South Basildon and East Thurrock and John Baron MP for Basildon and Billericay.
Following are some of the key reactions to Tuesday night's huge defeat for British Prime Minister Theresa May.
"If a government can not get its legislation through Parliament, it must go to the country for a new mandate", Corbyn said ahead of the vote, scheduled for 7 p.m. (1900GMT, 2 p.m. EST).
After the results of the confidence vote were announced to cheers from Conservative politicians, Mrs May told the House of Commons that Parliament had a duty to find a solution that would deliver Brexit.
The vote was tabled by the leader of the opposition Labour Party Jeremy Corbyn on Tuesday evening moments after Members of Parliament heavily voted down the government's Brexit deal, 432-202.
"It would bring division when we need unity, it would bring chaos when we need certainty and it would bring delay when we need to move forward", she said.
European leaders are now preparing for the worst - even though German Chancellor Angela Merkel said there was still time for further Brexit talks. Pro-Brexit lawmakers say the deal will leave Britain bound indefinitely to European Union rules, while pro-EU politicians favor an even closer economic relationship with Europe.
The way forward for Brexit is not yet clear and May's options are now limited, given that the Brexit deal she was offering was voted down so dramatically on Tuesday.
The UK's political process gives the executive unique powers to control how parliament does business, and at the same time Brexit has been such a divisive issue in the UK that alternatives have been hard to advance - enabling her to set the agenda unopposed.
Why did MPs reject Theresa May's deal?
"The EU I think isn't interested in any further renegotiation on this exit deal", he said. May's deal is still the "basis" for reaching that consensus, she said.
Were these discussions to have taken place after the 2017 election, or when May became prime minister, agreement might just have been possible - but at this late stage, and with May appearing to be unwilling to compromise in any meaningful way, cross-party agreement looks unattainable.
But with no clear majority in Parliament for any single alternative on Brexit, there's a growing chance that Britain may seek to postpone its departure date while politicians work on a new plan - or even hand the decision back to voters in a new referendum on Britain's European Union membership.
The U.K.is rapidly running out of time before the March 29 deadline.
It was the biggest defeat for a government in modern British history.
The prime minister is set to head back to Brussels in yet another attempt to secure concessions from the European Union on the deal - something EU Commission president Jean-Claude Juncker has repeatedly said he is not willing to do. But Mr. Corbyn believes staying in the customs union would protect jobs and help United Kingdom businesses.
But Mr Corbyn responded: "Before there can be any positive discussions about the way forward, the Government must remove clearly once and for all the prospect of the catastrophe of a no-deal Brexit from the European Union and all the chaos that would come as a result of that".
"A more ambitious future relationship would be positive for both sides and we're ready to engage on it in a constructive way", he added. "What yesterday's vote showed is that the political conditions for the ratification of the withdrawal agreement are not yet there in London".