Shaw, who started his first match for England since March 2017, suffered a blow to his head as he landed awkwardly after a challenge against Spain's Dani Carvajal, which saw him substituted after 57 minutes.
England's midfielder Dele Alli (C) and England's midfielder Eric Dier (2nd L) speak with Dutch referee Danny Makkelie after he disallows a late goal by England's striker Danny Welbeck (not pictured) during the UEFA Nations League football match between England and Spain at Wembley Stadium in London on September 8, 2018.
Manchester United forward Marcus Rashford opened scoring for the Three Lions but a goal each from Saul Niguez and Rodrigo ensured the 2010 World Cup winners emerge victorious.
Shaw was the world's most expensive teenager when he signed for United from Southampton for a reported £27 million ($35 million) in 2014, weeks after representing England at the World Cup in Brazil.
Shaw has only played seven minutes of worldwide football in the three years since as he has struggled to hold down a place in the United team until the start of this season.
"Spain came from behind to win against England and did so by showing the same personality as their coach". The FA's chief executive took the slightly perplexing step of publicly warning his highest-profile employee that he should not expect top Premier League money, but he stated his desire to talk on Southgate's return from holiday.
But he does not appear to be rushing to commit to England beyond the next European Championship and when so much progress has been made under his leadership, that should be considered a concern.
England must wait until October to attempt to make up lost ground in the Nations League with games against Croatia and Spain, but before then they face Switzerland in a friendly.
"We knew it was going to be a hard game". "We have to give the other players a start", the manager said. He is a player who, one way or another, ends up on the winning side. "I've seen teams fold in similar circumstances". We have two of the world's best teams in our group.