The Republican president said they must agree to "fix the bad flaws of the Iran nuclear deal", which was agreed to under his Democratic predecessor, Barack Obama, or he would refuse to extend USA sanctions relief on Iran.
"We will not be the first to violate the accord, but they should definitely know that they will regret it if they violate it", Rouhani said in a conference in Tehran marking National Nuclear Technology Day.
Gholamreza Ansari, a member of the Tehran City Council, said on Monday that the USA will be harmed if it withdraws from the 2015 nuclear deal.
In January, Trump announced he is waiving the United States sanctions against Iran for the "last time". "Our response will be stronger than what they imagine and they would see that within a week", Rouhani is quoted as saying.
During a ceremony held on Monday to mark Iran's National Day of Nuclear Technology, President Hassan Rouhani issued an order to transfer yellowcake produced in the Ardakan factory, located in the central province of Yazd, to a uranium conversion facility in Isfahan.
In 2015, Iran and a group of world powers agreed to substantially curb Tehran's nuclear development in return for lifting economic sanctions.
Rouhani said on Monday that Iran's missile capabilities were purely defensive.
The bodies of the Iranians, described as military advisers, had been flown back to Iran and funerals would take place later on Tuesday, Tasnim said.
Tehran and Moscow are completely unanimous in many regional fields, he said, adding, "It is understandable for us that some Western countries are annoyed with Russian Federation and are trying to make problems for this great power in the region and the world".
"Iran's stance on chemical weapons is clear and we condemn any use of them against any target", Zarif added.
On Sunday, Iran's national currency the Rial plunged above 6 percent against the United States dollar in free market.
The rial fell to a record low of 57,000 against the dollar by late afternoon on the unregulated currency market, according to the semiofficial Iranian Students' News Agency. A dollar was 36,000 rials in mid-September.