"The US are actually covering the Isis combat units to recover their combat capabilities, redeploy, and use them to promote the American interests in the Middle East", the statement concluded.
Earlier on Tuesday, the ministry posted a statement on its Facebook page saying the US air force had tried to hinder Russian strikes on Islamic State militants, accompanied by satellite photographs it said showed a vehicle convoy of Islamic State militants leaving the Syrian town of Albu Kamal on November 9 2017.
The Defense Ministry removed the photos from the post on Facebook and deleted the tweet.
The ministry also said that the USA coalition categorically refused to strike the retreating terrorists in spite of repeated proposals by the Russian military to conduct joint actions.
The allegations are extremely grave, but may be harder to take seriously given the "irrefutable proof" offered in the form of photographic accompaniment.
Another image purporting to show the same convoy in Syria was actually taken from a 2016 video published by Iraq's defence ministry, which said it was an Islamic State convoy fleeing Fallujah, Iraq, Conflict Intelligence Team investigators found.
AFP was able to compare the images in the emailed defence ministry statement with the images of the videogame on YouTube and confirm the resemblance.
A cached version of the original statement can still be viewed online.
The ministry said it would investigate the incident, which it said was caused by a civilian employee, but did not say how or why the pictures used were incorrect.
The militants had been forced to retreat from the city of Abu Kamal in the Syrian province of Deir al-Zour, one of the last remaining IS strongholds in Syria, by the advancing government troops.
Moscow has previously accused Washington of "pretending" to fight IS in Iraq, allowing jihadists into neighboring Syria where Russian Federation and the United States have backed opposing sides in the bloody conflict. A scene in a documentary about Putin made by the U.S. film-maker Oliver Stone showed Putin playing the director video footage on a telephone of what he said were Russian forces on operations in Syria.